What is a CyQUANT assay?
The CyQUANT™ Cell Proliferation Assay is a highly sensitive fluorescence-based method for quantifying cells and assessing cell proliferation and cytotoxicity. For cell proliferation assays that are more compatible with high throughput screening, see our CyQUANT™ NF Cell Proliferation Assay or our CyQUANT™ Direct Assay.
How does CyQUANT work?
CyQUANT GR dye binds to cellular nucleic acids, allowing cell numbers to be calculated from a standard curve. Because DNA-to-RNA ratios can vary over the course of the cell cycle, the CyQUANT kit allows users to determine cell numbers using RNase-digested cell lysates and a nucleic acid standard curve.
How do I do an MTT assay?
Assay protocol
- Discard media from cell cultures.
- Add 50 µL of serum-free media and 50 µL of MTT solution into each well.
- Incubate the plate at 37°C for 3 hours.
- After incubation, add 150 µL of MTT solvent into each well.
- Wrap plate in foil and shake on an orbital shaker for 15 minutes.
- Read absorbance at OD=590 nm.
What does MTS assay measure?
The MTS assay is used to assess cell proliferation, cell viability and cytotoxicity. The MTS assay protocol is based on the reduction of the MTS tetrazolium compound by viable mammalian cells (and cells from other species) to generate a colored formazan dye that is soluble in cell culture media.
Why MTT assay is done?
The MTT assay is used to measure cellular metabolic activity as an indicator of cell viability, proliferation and cytotoxicity.
What is MTT stand for?
MTT
| Acronym | Definition |
|---|---|
| MTT | Mean Transit Time (brain tissue blood flow) |
| MTT | Mobile Training Team |
| MTT | Molecular Targeted Therapies (oncology) |
| MTT | Multi-Table Tournament (poker) |
What is the working principle of the MTT assay?
Principle: Rapid colorimetric assay based on the cleavage of the tetrazolium ring of MTT (3-(4,5-dimethylthazolk-2-yl)-2,5-diphenyl tetrazolium bromide) by dehydrogenases in active mitochondria of living cells as an estimate of viable cell number.
What is the difference between MTT and MTS assay?
Definition. MTT assay refers to a colorimetric assay for assessing cell metabolic activity while MTS assay refers to a ‘one-step’ MTT assay which offers the convenience of adding the reagent straight to the cell culture without the intermittent steps required in the MTT assay.
How do you analyze MTS assay?
Calculations involved:
- Subtract the absorbance of the blank wells from all the wells.
- Divide the absorbance of the wells which have the cells treated with the drug/inhibitor by the average of the absorbances emitted from the cells in the control wells.
- Multiply the ratio by 100 to give you the viability in %.
What does a high LDH mean?
Having higher-than-normal LDH levels usually means you have some type of tissue damage from an injury, disease or infection — whether chronic or acute. Conditions that cause high LDH levels include: Anemia. Kidney disease. Lung disease.
Is LDH assay good?
This LDH cytotoxicity assay is capable of detecting low level damage to cell membrane which cannot detected using other methods. And since it does not damage healthy cells, the LDH cytotoxicity assay can be performed directly in the cell culture wells containing a mixed population of viable and damaged cells.
What is cyquant® direct assay?
The CyQUANT® Direct assay is a highly sensitive and robust method to assess cell growth, viability, or compound toxicity in a range of applications, from high throughput screening to bioproduction.
What is the cyquant LDH cytotoxicity assay?
The CyQUANT LDH Cytotoxicity Assay provides the reagents to accurately and quantitatively measure this extracellular LDH. Note: The CyQUANT LDH Cytotoxicity Assay (Cat. Nos. C20300 and C20301) are direct replacements for the Pierce LDH Cytotoxicity Assay Kit (Cat. Nos. 88953 and 88954).
What is the difference between colorimetric-based and cyquant-based assays?
CyQUANT Cell Proliferation Assays are ideal for high-throughput screening, are more sensitive than colorimetric-based assays, and are not radioactive. Rapid and easy to use, CyQUANT Cell Proliferation Assays Kits require no washes, extractions, growth medium changes, or long incubations, and don’t rely metabolic status of the cell.
What is the fluorescence range of the cyquant NF cell proliferation assay?
Quantitation of CHO (M1WT3) cells using the CyQUANT NF Cell Proliferation Assay Kit. Fluorescence measurements were made using a microplate reader with excitation at 485 nm and emission detection at 530 nm. The linear range of the assay under these conditions is from 100 to 20,000 cells per 100 µL sample.