Which stain is used for staining reticulin Fibres?
Ammoniacal silver stains
Ammoniacal silver stains are most commonly used for reticular fiber and basement membrane staining.
How do you stain reticulin?
Reticulin fibers are agyrophilic, meaning that these tissue elements will stain black with a silver solution using the aid of a chemical reducer, which brings the silver into a visible form. This silver staining process is known as silver impregnation.
For which purpose reticulin stain is done?
Stain of reticulin is a staining that stains the reticulinic fibers of the stroma and makes it possible to specify the architecture of the tumors (as for example the follicular architecture). It is very useful in cases where the tumor is necrotic.
What is gomori method?
Gomori (1939) described a method of demonstrating alkaline phosphatase. activity in tissue slides. This method consists in incubating the fixed tissue. slides with buffered solution of a phosphate ester and a calcium salt.
What does gomori trichrome stain?
Gomori Trichrome Stain is a histological stain used on muscle tissue and can be used to test for certain forms of mitochondrial myopathy. Gomori Trichrome Stain will stain muscle fibers and cytoplasm in red, nuclei in black and collagen containing connective tissues in blue.
Which method is used to demonstrate reticulin fibers?
Gordon and Sweet’s silver staining method
The Gordon and Sweet’s silver staining method is used to demonstrate reticular (retic) fibers. This method relies on the impregnation of retic fibers with silver through oxidation and reduction. The tissue is first oxidized using potassium permanganate to enhance subsequent staining.
Which stain is best for glycogen?
The selective staining of glycogen by Best’s Carmine and the other dyes used in a similar manner (e.g. Alizarin Brilliant Blue BS, Alizarin Red S, Gallein and Haematoxylin) is due to hydrogen bonding between phenolate (−O−) groups of the dye and glycogen hydroxyl groups.
What is reticulin stain in bone marrow?
Reticulin is a normal component of the bone marrow stroma and can be detected with a reticulin stain in 73% to 81% of healthy subjects. 16-19. Increased reticulin staining (reticulin fibrosis) is associated with many benign conditions as well as some malignant diseases.
How does gomori trichrome stain work?
The principle of Gomori trichrome staining is based on single-step staining. A plasma dye (2R chromotropic) and connective tissue fiber dye (fast green FCF, light green or aniline blue) are combined in a solution of phosphotungstic acid in which glacial acetic acid is added.
What is GMS Stain used for?
Grocott methenamine silver (GMS) stain is commonly used for the identification of fungi on cytosmears and tissue sections. It imparts a black color to the fungal profiles and a pale green color to the background. It stains all pathogenic and nonpathogenic fungi and melanin.
What is Van Gieson stain used for?
Van Gieson’s trichrome is used for the differentiation between collagen and smooth muscle in tumors and to demonstrate the increase in the amount of collagen in these pathologies.
What stain is used to stain collagen?
Mason’s Trichrome stain is used to highlight connective tissue fibers in a tissue section. Collagen fibers are stained a blue hue, nuclei a blue-black, and cytoplasm, keratin, and muscle fibers a range of pink to red hues. AZAN Trichrome stain is used to highlight collagen fibers within a tissue section.
What is Gomori’s one-step trichrome?
PRINCIPLE: Gomori’s one-step trichrome is a staining procedure that combines the plasma stain (chromotrope 2R) and connective fiber stain (fast green FCF) in a phosphotungstic acid solution to which glacial acetic acid has been added.
What is gomorri’s technique?
Gomorri’s technique involves the impregnation of silver for the demonstration of reticular fibres. Since the reticular fibres have low affinity for silver salts, a suitable pretreatment in order to enhance the selectivity to impregnation is required.
What is a reticular fibre?
Reticular fibre is the type of connective tissue composed of type III collagen secreted by reticular cells. Gomorri’s technique involves the impregnation of silver for the demonstration of reticular fibres.
How to increase the selectivity of silver salts to impregnation in reticular fibres?
Since the reticular fibres have low affinity for silver salts, a suitable pretreatment in order to enhance the selectivity to impregnation is required. Treatment with silver solution has two fold effects :