What is the wavelength for Cy3?
around 548-552 nm
Cy®3 is an orange fluorophore commonly used in applications such as immunolabeling, nucleic acid labeling, fluorescence microscopy, and flow cytometry. The dye has an absorption wavelength that peaks around 548-552 nm, and an emission maximum around 562-570 nm.
What does Cy3 stain for?
The Cy3 dye is a traditional orange-fluorescent label for protein and nucleic acid conjugates used for imaging, flow cytometry, and genomic applications. It is also the basis for the classic lipophilic tracer DiI and its variants.
What color does Cy3 fluorescence?
greenish yellow
Cy3 fluoresces greenish yellow (~550 nm excitation, ~570 nm emission), while Cy5 is fluorescent in the far-red region (~650 excitation, 670 nm emission). Cy3 can be detected by various fluorometers, imagers, and microscopes with standard filters for Tetramethylrhodamine (TRITC).
What is Cy3 and Cy5?
The cyanine dyes Cy3 and Cy5 are among the most widely used and versatile8 oligonucleotide labels in, e.g., microarray experiments, fluorescent in situ hybridization (FISH), real-time PCR (RT-PCR), and FRET studies9,10 and are considered to be relatively environmentally insensitive.
What does Cy3 stand for?
Cy3 (Sulfo-Cyanine3) is an orange-fluorescent label for protein and nucleic acid (λex=554, λem=568).
How does Alexa Fluor work?
Alexa Fluor dyes can be conjugated directly to primary antibodies or to secondary antibodies to amplify signal and sensitivity or other biomolecules. The excitation and emission spectra of the Alexa Fluor series cover the visible spectrum and extend into the infrared.
Is Cy3 pH sensitive?
Cy3 excites maximally at 544 nm and has an emission peak at 564 nm (Figure 3B), and produces a pH-insensitive synchronous scan peak at 548 nm (Figure 4).
Can you use Cy3 and Cy5 together?
By separately imaging two chromatically different fluorophores (Cy3 and Cy5) conjugated to a single molecule, we are able to localize both probes simultaneously.
What Cy3 and Cy5 denotes in microarray technology?
The microarray is analyzed in a scanner, which uses laser light to cause each spot to fluoresce and measures the relative intensity of emission, typically at green (Cy3) and red (Cy5) wavelengths.
How long do Alexa Fluor antibodies last?
2 weeks
What temperature should I store the antibody at? Storage at 4°C should not exceed 1 or 2 weeks.
Is Alexa Fluor 488 same as FITC?
Alexa Fluor 488 (Ex-Max 495 nm/Em-Max 519 nm): have nearly identical emission and excitation maxima as FITC. However, Alexa Flour 488 display higher photostability and is less susceptible to self-quenching.
What does Texas red stain?
Texas Red or sulforhodamine 101 acid chloride is a red fluorescent dye, used in histology for staining cell specimens, for sorting cells with fluorescent-activated cell sorting machines, in fluorescence microscopy applications, and in immunohistochemistry.