How do I dissolve EDC?
For BSA or OVA conjugation, dissolve 10mg of EDC in 1mL of ultrapure water and immediately add 100µL of this solution to the carrier-peptide solution. For KLH conjugation, dissolve 10mg of EDC in 1mL of ultrapure water and immediately add 50µL of this solution to the carrier-peptide solution.
What is EDC HCl?
1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC, EDAC or EDCI) is a water-soluble carbodiimide usually handled as the hydrochloride. It is typically employed in the 4.0-6.0 pH range. It is generally used as a carboxyl activating agent for the coupling of primary amines to yield amide bonds.
What is EDAC used for?
EDAC HCl is used for modifying NMDA receptors and is also used as a condensing agent in the synthesis of peptides. It is also being used increasingly in the production of new pharmaceuticals due to its simple purification process.
Is EDC a carbodiimide?
Yes, both acronyms are used for the same compound. The chemical name for EDC is 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide and the chemical name for EDAC is N-3-(3-dimethylaminopropyl)-N-ethyl-carbodiimide. Both of these compounds are structurally equivalent.
Is EDC soluble in water?
Since EDC hydrochloride is water soluble, it is a viable component to use in the preparation of chloroanthraquinone hydrazides.
What is EDC and NHS?
EDC, in conjunction with NHS allows, for 2-step coupling of two proteins without affecting the carboxyls of the second protein. First, EDC activates carboxyl groups and forms an amine reactive O-acylisourea intermediate that spontaneously reacts with primary amines to form an amide bond and an isourea by-product.
Does EDC react with water?
Hello Kimi, EDC could be used in water, but the procedure is different (typically in combination with NHS). The reaction is quite sensitive to pH.
What is EDC product?
Electronic Data Capture (EDC) is a software product that is utilized by Life Sciences Organizations who perform research studies in a regulated environment.
Which catalyst is used in EDC manufacturing?
The high temperature process utilises the heat of reaction in the distillation of the EDC , leading to considerable energy savings. oxygen, are reacted at 200-300°C and 4-6 bar in the presence of a catalyst, usually cupric chloride.