In some occasions, you will have to write an essay in the extremely short amount of time on the exam in college or high school. Also, you may be a little bit of a procrastinator, and find yourself in a situation when the paper is due tomorrow morning, and you have not even chosen the topic yet. Even though a last-minute essay cannot look as great as a work prepared successively and carefully within the whole time given, you still have a chance to submit a decent paper. The working process will require your full attention and a lot of effort, even if you are assigned a simple essay. However, if you learn the next few tips, the essay writing will seem significantly easier and feasible even when you are short on time.

Firstly, clean up your working space to get started. Make sure you have everything you need on the table, take a pen, a few sticky notes, your laptop, and read through the assignment requirements. In case no prompt is given, search for good essay topics, and pick a few uncommon and interesting ones you will be able to write about. Making a final choice, think which topic is the most relevant to your current studies and will not take too much to research.

Afterwards, look for the most trustworthy sources or the ones you are certainly allowed to use. If you are not sure, access the online library or any free services where you can look for the books and articles for your essay. Use sticky notes to write down the information and put them in front of you to see how much data has been gathered and if you need to continue researching. Reread these notes from time to time and cross out the info you do not find relevant anymore.

When you have the data you need to produce a quality work, it is crucial to think about the structure of the future paper. If you are not sure how to write an essay outline properly, check what your essay type is first. Each type is organized differently, so you need to look up the structure every time you are given an essay homework. You can also search for an example of the essay on your topic, and adhere to its outline. No matter what kind of essay you are going to write, it is important to start with a thesis statement. It should declare what problem you will review in the paper, and which facts or arguments you will use to do it professionally. As these arguments will be discussed in the main part of the essay, outline the body paragraphs and put down a few sentences with the rough description of each paragraph. Think of the way you will engage the reader in the introduction, and which thought will be conclusive for the paper. When the direction of the work is clear from the outline, use it to draft the first version of the essay.

If you are not used to model essay writing, do not worry - your draft should not necessarily look like a masterpiece. It is only the depiction of your thoughts, and as you will have them written down, it will be easier to create a good essay. There is no best way to write an essay, so trust the working methods you usually use. You may like taking short breaks once in a few minutes, or write everything in one sit - just make sure to keep the focus on writing and avoid the urge to call a friend or watch something online. Thus, you will finish the paper faster, and will not feel guilty for engaging in other activities afterwards.

Do not forget to go through the essay a few times after the completion. Everyone makes typos and mistakes by accident, but it is about you to find and fix them before your teacher does. If you need help with an essay editing, try asking a friend or a family member to read and analyze your work. Also, you can order editing services in case your paper needs to be perfectly polished so that you can submit an ideal essay and get an excellent grade.

As these steps are simple to follow, you will not have any problems coping with an essay on time. Try the whole procedure at least once, and you will not have to use any other tips preparing an essay paper during your studies!

How does Roche 454 sequencing work?

Roche 454 sequencing can sequence much longer reads than Illumina. Like Illumina, it does this by sequencing multiple reads at once by reading optical signals as bases are added. As in Illumina, the DNA or RNA is fragmented into shorter reads, in this case up to 1kb.

What is 454 sequencing used for?

In 2005, 454 Life Sciences launched the first next-generation DNA sequencer – a big leap forward in DNA sequencing technology. The 454 method can sequence fragments of DNA? equivalent to up to one billion bases?, in a single day (that’s a 1/3 of the human genome?).

Why is it called 454 sequencing?

For their method for low-cost gene sequencing, 454 Life Sciences was awarded the Wall Street Journal’s Gold Medal for Innovation in the Biotech-Medical category in 2005. The name 454 was the code name by which the project was referred to at CuraGen, and the numbers have no known special meaning.

What is the principle of Roche 454?

Its main principle of sequencing is illustrated as follows. DNA Library construction in 454 sequencing system is different from that of Illumina. It uses spray method to break DNA samples into small fragments of 300-800bp, and adds different adapters at both ends.

How is 454 data generated?

The 454 Sequencing™ process uses a sequencing by synthesis approach to generate sequence data. In sequencing by synthesis, a single-stranded DNA fragment is copied with the use of an enzyme making the fragment double stranded.

What are different types of enzymes used in the process of 454 pyrosequencing?

Pyrosequencing is a real-time method catalyzed by four kinetically well-balanced enzymes: DNA polymerase, ATP sulfurylase, firefly luciferase and apyrase.

How is next-generation sequencing performed?

How NGS Works. The basic next-generation sequencing process involves fragmenting DNA/RNA into multiple pieces, adding adapters, sequencing the libraries, and reassembling them to form a genomic sequence. In principle, the concept is similar to capillary electrophoresis.

How many colors of light are used in pyrosequencing?

There is just one color because the bases are washed across one at a time and there is only one enzyme doing the fluorescing. 2. Describe the activities that take place during the pyrosequencing reaction (enzymes, beads, and substrates).

How many types of beads are used in 454 sequencing?

There are three types of beads. One type is the primer coated capture beads that capture the original strand of DNA and are the base of replication for emPCR. The second type of bead is enzyme coated with ATP Sulfurylase and Luciferase to create the fluorescent light used for base detection.

Does pyrosequencing require a primer?

Pyrosequencing has potential advantages of accuracy, flexibility, parallel processing, and can be easily automated. Furthermore, the technique avoids the need for labeled primers, labeled nucleotides, and gel electrophoresis.

What is next generation sequencing platform of Roche 454?

The Next Generation Sequencing Platform of Roche 454 Roche 454 Roche 454 sequencing system is the first commercial platforms for the next generation sequencing technology. Its main principle of sequencing is illustrated as follows.

What is the difference between Roche 454 and Illumina DNA amplification?

Of course, DNA amplification process of Roche 454 system is different from that of Illumina. These single stranded DNAs would be fixed by 28um beads which are buried in emulsion. The biggest feature of emulsion PCR is the formation of a large number of independent reaction space for DNA amplification.

What is the principle of 454 sequencing?

Roche 454 sequencing system is the first commercial platforms for the next generation sequencing technology. Its main principle of sequencing is illustrated as follows. a. Preparation of DNA Library DNA Library construction in 454 sequencing system is different from that of Illumina.

What is sureselect target enrichment system for Roche 454?

SureSelect Target Enrichment System for Roche 454 GS FLX and GS Junior Sequencing Platforms Protocol Version 1.4.1, August 2015 SureSelect platform manufactured with Agilent SurePrint Technology For Research Use Only. Not for use in d iagnostic p rocedures. 2 SureSelect Target Enrichment System for Roche 454 Titanium Sequencing Libraries Notices