How will the arrangement of the Lactococcus lactis?
How will the arrangement of the Lactococcus lactis in broth differ from the arrangement in the slant culture? In the broth, the bacteria will grow in a small area and are closer together. In the slant culture, the bacteria will be more spread out.
Why was the arrangement of lactococcus from the broth culture different than that from the slant culture in the second period?
Why was the arrangement of lactococcus from the broth culture, different than that from the slant culture in the second period? – In the broth they were able to grow freely and on top of each other. – In the slant they were able to grow in a way that shows binary fission on one plane.
Did growth occur at different levels in the agar deep?
Yes, growth did occur at different levels in the agar deep.
What is the primary use of broth cultures?
Broth cultures are a method of growing bacteria in a liquid growth medium. They’re used to grow and maintain cultures for a laboratory. Different bacteria may grow differently in broth cultures.
Can Lactococcus grow on MRS agar?
MRS medium is selective for lactobacilli but some growth of leuconostocs and pediococci may occur. Selectivity can be altered by pH adjustment. Lactobacilli will tolerate lower pH levels than streptococci (pH 5.0-6.5) with pediococci and leuconostocs growing best within this range.
Is Lactococcus lactis Gram-positive or negative?
Gram-positive
Lactic Acid Bacteria | Lactococcus lactis They are Gram-positive, catalase-negative, facultatively anaerobic, nonmotile, and non-spore-forming.
What is the primary use of slants and why?
What is the primary use of slants? Provides a solid growth area that is easier to store.
What is a slant used for quizlet?
An agar slant is a test tube of nutrient agar that was allowed to solidify at an angle creating a larger flat surface for growth at the top of the agar. Agar slants are most useful for growing maintenance cultures of microbes that we want to have on hand for extended periods.
What is the purpose of the slanted surface in an agar slant?
Slanting the surface of the agar gives the bacteria a greater surface area on which to grow in a test tube. Furthermore, slants are created in test tubes that can be capped, which minimizes water loss. This is important because of the high moisture content of agar media.
What is the use of agar slant and agar deep?
Agar slants are commonly used to generate stocks of bacteria. Agar plates can be used to separate mixtures of bacteria and to observe colony characteristics of different species of bacteria (you will perform an experiment in this lab to illustrate this).
Why are agar slants better for stock cultures?
Why are agar slants better suited than agar plates to maintain stock cultures? Slants are better suited because they can be capped, preventing the agar and culture from drying out. The cap also prevents airborne contaminates from entering the slant. Slants take up less storage space.
Why are slants preferred for the storage of bacteria?
Slanting the surface of the agar gives the bacteria a greater surface area on which to grow in a test tube. Furthermore, slants are created in test tubes that can be capped, which minimizes water loss.