What is an indican test?
The indican test is a quick and efficient screening exam that assays the possibility of dysbiosis using a patient’s urine. During occasions of overgrowth, bacteria intercept the amino acid tryptophan before it can be processed by the small intestine.
What is indican urine test?
The urine test for indican (also called the Obermeyer test) is an indicator of intestinal dysbiosis and small intestinal bacterial overgrowth (SIBO). Indican is a by-product (chemically an indole) produced when intestinal bacteria react with the amino acid tryptophan.
What color indicates a high positive indican test?
6. Compare the color in the bottom chloroform layer with the Indican Color Chart below. Record results and dispose the materials properly. Clear or Urine color 0: Normal Light Blue 1: Low Positive Blue 2: Medium Positive Violet 3: High Positive Jet Black 4: Very High Positive.
What is the use of the Obermeyer’s test?
The urinary Indican (Obermeyer) test is an accurate, simple, fast (15 min) and inexpensive screening tool for the identification of protein putrefaction in the gastrointestinal tract. Indican levels are directly associated with bacterial activity in the intestines.
What is the significance of indican?
Indican is a tryptophan metabolite that is excreted mostly in the feces but also in small amounts in the urine as a result of absorption and detoxification of indole produced by bacterial action on tryptophan in the intestines. The presence of indican in the urine indicates amino acid malabsorption.
How is indican formed?
Indican is produced from tryptophan in a manner reminiscent of the production of p-cresol sulfate from tyrosine and phenylalanine. Gut bacteria convert tryptophan to indole, which is then oxidized to indoxyl and conjugated with sulfate in the liver.
What is the other name of indican?
Alternative name: potassium indoxylsulphate; chemical formula C₈H₆NOSO₂OH. ‘We have studied the effect of indican on six commercial procedures for the measurement of total bilirubin in serum. ‘
What conditions may lead to increased concentration of indican in the urine?
Excessive Indican levels are caused by poor digestion, constipation, exposure to excessive chemicals in the water, food, air, or drugs, or yeast/candida, parasites, or abnormal flora in the gut.
What are the conditions that result in high or increased Indican levels in urine?
Urinary concentrations of indole and indican, which are degradation products of tryptophan, are increased in patients with small intestinal bacterial overgrowth (SIBO). These changes, associated with bacterial overgrowth can contribute to the dysfunction of mucosal cells, impairing protein and carbohydrate absorption.
Which of the following is putrefaction product from the bacterial Deconjugation of tryptophan to indole in the small intestines?
INDICAN
INDICAN (indoxyl sulfate) is a putrefaction product resulting from bacterial deconjugation of dietary tryptophan to indole in the small intestine. Indican levels are directly associated with bacterial activity in the intestines.
How is indican made?
Indican is an indole produced when bacteria in the intestine act on the amino acid, tryptophan. Most indoles are excreted in the feces. The remainder is absorbed, metabolized by the liver, and excreted as indicanin the urine.
What is indican level?
Urinary indican levels are a measure of intestinal putrefaction. Intestinal putrefaction by-products that are excreted via the kidneys are presumably absorbed via intestinal circulation. All intestinal circulation enters into portal circulation prior to entrance into pulmonary and then systemic circulation.