In some occasions, you will have to write an essay in the extremely short amount of time on the exam in college or high school. Also, you may be a little bit of a procrastinator, and find yourself in a situation when the paper is due tomorrow morning, and you have not even chosen the topic yet. Even though a last-minute essay cannot look as great as a work prepared successively and carefully within the whole time given, you still have a chance to submit a decent paper. The working process will require your full attention and a lot of effort, even if you are assigned a simple essay. However, if you learn the next few tips, the essay writing will seem significantly easier and feasible even when you are short on time.

Firstly, clean up your working space to get started. Make sure you have everything you need on the table, take a pen, a few sticky notes, your laptop, and read through the assignment requirements. In case no prompt is given, search for good essay topics, and pick a few uncommon and interesting ones you will be able to write about. Making a final choice, think which topic is the most relevant to your current studies and will not take too much to research.

Afterwards, look for the most trustworthy sources or the ones you are certainly allowed to use. If you are not sure, access the online library or any free services where you can look for the books and articles for your essay. Use sticky notes to write down the information and put them in front of you to see how much data has been gathered and if you need to continue researching. Reread these notes from time to time and cross out the info you do not find relevant anymore.

When you have the data you need to produce a quality work, it is crucial to think about the structure of the future paper. If you are not sure how to write an essay outline properly, check what your essay type is first. Each type is organized differently, so you need to look up the structure every time you are given an essay homework. You can also search for an example of the essay on your topic, and adhere to its outline. No matter what kind of essay you are going to write, it is important to start with a thesis statement. It should declare what problem you will review in the paper, and which facts or arguments you will use to do it professionally. As these arguments will be discussed in the main part of the essay, outline the body paragraphs and put down a few sentences with the rough description of each paragraph. Think of the way you will engage the reader in the introduction, and which thought will be conclusive for the paper. When the direction of the work is clear from the outline, use it to draft the first version of the essay.

If you are not used to model essay writing, do not worry - your draft should not necessarily look like a masterpiece. It is only the depiction of your thoughts, and as you will have them written down, it will be easier to create a good essay. There is no best way to write an essay, so trust the working methods you usually use. You may like taking short breaks once in a few minutes, or write everything in one sit - just make sure to keep the focus on writing and avoid the urge to call a friend or watch something online. Thus, you will finish the paper faster, and will not feel guilty for engaging in other activities afterwards.

Do not forget to go through the essay a few times after the completion. Everyone makes typos and mistakes by accident, but it is about you to find and fix them before your teacher does. If you need help with an essay editing, try asking a friend or a family member to read and analyze your work. Also, you can order editing services in case your paper needs to be perfectly polished so that you can submit an ideal essay and get an excellent grade.

As these steps are simple to follow, you will not have any problems coping with an essay on time. Try the whole procedure at least once, and you will not have to use any other tips preparing an essay paper during your studies!

What is an LNA oligo?

Abstract. Locked nucleic acid (LNA) is the term for oligonucleotides that contain one or more nucleotide building blocks in which an extra methylene bridge fixes the ribose moiety either in the C3′-endo (beta-D-LNA) or C2′-endo (alpha-L-LNA) conformation.

What are oligo probes?

Definition. Oligonucleotide probes are short stretches of single-stranded DNA or RNA used to detect the presence of complementary nucleic acid sequences (target sequences) by hybridization.

What are locked nucleic acid probes?

Locked nucleic acids are modified RNA monomers. The “locked” part of their name comes from a methylene bridge bond linking the 2′ oxygen to the 4′ carbon of the RNA pentose ring (Figure 1). The bridge bond fixes the pentose ring in the 3′-endo conformation.

What are the two types of DNA probes?

Gene probes can be produced in several ways and fall broadly into three types: gene-specific probes, oligonucleotide probes and polymorphic probes. Gene-specific probes are produced from specific mRNA by the enzyme reverse transcriptase, which synthesizes a complementary DNA copy (cDNA) from mRNA.

What is LNA base?

A locked nucleic acid (LNA), also known as bridged nucleic acid (BNA), and often referred to as inaccessible RNA, is a modified RNA nucleotide in which the ribose moiety is modified with an extra bridge connecting the 2′ oxygen and 4′ carbon.

How are oligonucleotide probes produced?

Oligonucleotide probes are often prepared with covalent attachment of a reporter molecule (eg, a fluorescent dye) or affinity labels that allow them to be attached to solid supports. Probes used in homogeneous (real-time) PCR are usually oligonucleotides with a fluorescent label.

What is this oligonucleotide probe specifically called as?

Sequence-Specific Oligonucleotide Probe (SSOP) Typing. DNA from a panel of samples (96- or 384-well format) are PCR amplified using locus-specific primers. The PCR products are denatured and blotted on a series of nylon membranes and probed with a panel of allele/group-specific probes.

How many nucleotides are in a probe?

In molecular biology, a hybridization probe (HP) is a fragment of DNA or RNA of usually 15–10000 nucleotide long which can be radioactively or fluorescently labeled.

How do locked nucleic acid (LNA) DNA probes differ from oligonucleotide DNA probes?

Oligonucleotide probes containing locked nucleic acid (LNA) hybridize to complementary single-stranded target DNA sequences with an increased affinity compared to oligonucleotide DNA probes.

What is the optimal length of LNA substituted labeled probes?

Optimal length of LNA substituted labeled probes: 15-18 nucleotides (Please note that these are 5-8 bases shorter than the corresponding DNA probes). Maintain T m with LNA substitutions to match the T m of the corresponding longer DNA probes.

What is the composition of LNA in oligos?

LNA Oligos are composed of either 100% Lock Nucleic Acid (LNA) bases or a mixture of LNA and DNA bases. However, only oliognulceotides that are between 7 and 15 mers in length can be made up of 100% LNA.

What is a locked nucleic acid oligonucleotide used for?

Locked nucleic acid oligonucleotides are useful in template switching oligo designs and for strengthening target oligo binding in challenges sequence regions, such as AT-rich areas. As with locked nucleic acid qPCR probes, hybridization T m can be manipulated by the number of locked nucleic acid bases incorporated.