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In some occasions, you will have to write an essay in the extremely short amount of time on the exam in college or high school. Also, you may be a little bit of a procrastinator, and find yourself in a situation when the paper is due tomorrow morning, and you have not even chosen the topic yet. Even though a last-minute essay cannot look as great as a work prepared successively and carefully within the whole time given, you still have a chance to submit a decent paper. The working process will require your full attention and a lot of effort, even if you are assigned a simple essay. However, if you learn the next few tips, the essay writing will seem significantly easier and feasible even when you are short on time.

Firstly, clean up your working space to get started. Make sure you have everything you need on the table, take a pen, a few sticky notes, your laptop, and read through the assignment requirements. In case no prompt is given, search for good essay topics, and pick a few uncommon and interesting ones you will be able to write about. Making a final choice, think which topic is the most relevant to your current studies and will not take too much to research.

Afterwards, look for the most trustworthy sources or the ones you are certainly allowed to use. If you are not sure, access the online library or any free services where you can look for the books and articles for your essay. Use sticky notes to write down the information and put them in front of you to see how much data has been gathered and if you need to continue researching. Reread these notes from time to time and cross out the info you do not find relevant anymore.

When you have the data you need to produce a quality work, it is crucial to think about the structure of the future paper. If you are not sure how to write an essay outline properly, check what your essay type is first. Each type is organized differently, so you need to look up the structure every time you are given an essay homework. You can also search for an example of the essay on your topic, and adhere to its outline. No matter what kind of essay you are going to write, it is important to start with a thesis statement. It should declare what problem you will review in the paper, and which facts or arguments you will use to do it professionally. As these arguments will be discussed in the main part of the essay, outline the body paragraphs and put down a few sentences with the rough description of each paragraph. Think of the way you will engage the reader in the introduction, and which thought will be conclusive for the paper. When the direction of the work is clear from the outline, use it to draft the first version of the essay.

If you are not used to model essay writing, do not worry - your draft should not necessarily look like a masterpiece. It is only the depiction of your thoughts, and as you will have them written down, it will be easier to create a good essay. There is no best way to write an essay, so trust the working methods you usually use. You may like taking short breaks once in a few minutes, or write everything in one sit - just make sure to keep the focus on writing and avoid the urge to call a friend or watch something online. Thus, you will finish the paper faster, and will not feel guilty for engaging in other activities afterwards.

Do not forget to go through the essay a few times after the completion. Everyone makes typos and mistakes by accident, but it is about you to find and fix them before your teacher does. If you need help with an essay editing, try asking a friend or a family member to read and analyze your work. Also, you can order editing services in case your paper needs to be perfectly polished so that you can submit an ideal essay and get an excellent grade.

As these steps are simple to follow, you will not have any problems coping with an essay on time. Try the whole procedure at least once, and you will not have to use any other tips preparing an essay paper during your studies!

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What is iTaq Universal SYBR Green supermix?

What is iTaq Universal SYBR Green supermix?

iTaq Universal SYBR Green Supermix is a 2x concentrated, ready-to-use reaction master mix optimized for dye-based quantitative PCR (qPCR) on any real-time PCR instrument.

How does SYBR Green work in qPCR?

SYBR Green is one of the most commonly used fluorescent dyes in qPCR. It binds to double-stranded DNA molecules by intercalating between the DNA bases. Once intercalated to DNA, SYBR Green becomes less mobile, causing its energy to be released as fluorescence.

What is in SYBR Green Master Mix?

SYBR® Green Master Mix Components SYBR® Green master mixes are 2x concentrated, and contain dNTPs, MgCl2, and DNA polymerase. SYBR® Green master mixes are available with other dyes including ROX. Many PCR machines require a passive reference dye.

What is the difference between TaqMan and SYBR Green?

The main difference between STBR Green and Taqman is that SYBR green is a dsDNA binding dye used to detect PCR products accumulated during the PCR reaction whereas Taqman is a fluorogenic probe specific to a target gene, which accumulates during PCR.

Does SYBR Green inhibit PCR?

SYBR Green exhibits a very strong fluorescent signal, but it has been shown to inhibit the PCR reaction and has a narrow dynamic range and lower reproducibility than other detection chemistries [2, 3, 7].

What can be used as a substitute for SYBR Green?

EvaGreen
EvaGreen is another DNA dye which is less inhibitory to PCR than SYBR Green and is marketed as an alternative [12].

Is SYBR Green a master mix?

Applied Biosystems SYBR Green master mixes are designed for quantitative real-time PCR using a set of two PCR primers that flank the target region.

Can TaqMan probes be used with SYBR Green?

SYBR Green vs Taqman Taqman depend on hybridization probes and 5′ to 3′ exonuclease activity of Taq polymerase. No fluorescently labeled probes are not required. Dual-labeled probes are required.

Can TaqMan primers be used for SYBR Green?

Hi, Yes you can. We found the primers for EMCV in the publications too. It was designed for TaqMan, but in our research we use with SYBR green mastermix.

What is the difference between Taqman and SYBR Green?

What does SYBR stand for?

SYBR

Acronym Definition
SYBR Synergy Brands, Inc. (stock symbol)