In some occasions, you will have to write an essay in the extremely short amount of time on the exam in college or high school. Also, you may be a little bit of a procrastinator, and find yourself in a situation when the paper is due tomorrow morning, and you have not even chosen the topic yet. Even though a last-minute essay cannot look as great as a work prepared successively and carefully within the whole time given, you still have a chance to submit a decent paper. The working process will require your full attention and a lot of effort, even if you are assigned a simple essay. However, if you learn the next few tips, the essay writing will seem significantly easier and feasible even when you are short on time.

Firstly, clean up your working space to get started. Make sure you have everything you need on the table, take a pen, a few sticky notes, your laptop, and read through the assignment requirements. In case no prompt is given, search for good essay topics, and pick a few uncommon and interesting ones you will be able to write about. Making a final choice, think which topic is the most relevant to your current studies and will not take too much to research.

Afterwards, look for the most trustworthy sources or the ones you are certainly allowed to use. If you are not sure, access the online library or any free services where you can look for the books and articles for your essay. Use sticky notes to write down the information and put them in front of you to see how much data has been gathered and if you need to continue researching. Reread these notes from time to time and cross out the info you do not find relevant anymore.

When you have the data you need to produce a quality work, it is crucial to think about the structure of the future paper. If you are not sure how to write an essay outline properly, check what your essay type is first. Each type is organized differently, so you need to look up the structure every time you are given an essay homework. You can also search for an example of the essay on your topic, and adhere to its outline. No matter what kind of essay you are going to write, it is important to start with a thesis statement. It should declare what problem you will review in the paper, and which facts or arguments you will use to do it professionally. As these arguments will be discussed in the main part of the essay, outline the body paragraphs and put down a few sentences with the rough description of each paragraph. Think of the way you will engage the reader in the introduction, and which thought will be conclusive for the paper. When the direction of the work is clear from the outline, use it to draft the first version of the essay.

If you are not used to model essay writing, do not worry - your draft should not necessarily look like a masterpiece. It is only the depiction of your thoughts, and as you will have them written down, it will be easier to create a good essay. There is no best way to write an essay, so trust the working methods you usually use. You may like taking short breaks once in a few minutes, or write everything in one sit - just make sure to keep the focus on writing and avoid the urge to call a friend or watch something online. Thus, you will finish the paper faster, and will not feel guilty for engaging in other activities afterwards.

Do not forget to go through the essay a few times after the completion. Everyone makes typos and mistakes by accident, but it is about you to find and fix them before your teacher does. If you need help with an essay editing, try asking a friend or a family member to read and analyze your work. Also, you can order editing services in case your paper needs to be perfectly polished so that you can submit an ideal essay and get an excellent grade.

As these steps are simple to follow, you will not have any problems coping with an essay on time. Try the whole procedure at least once, and you will not have to use any other tips preparing an essay paper during your studies!

Thu. Apr 25th, 2024

Mixed

What percentage gel should I use Western blot?

What percentage gel should I use Western blot?

Table of Contents

Loading and running the gel

Protein size	Gel percentage
12–45 kDa	15%
10–70 kDa	12.5%
15–100 kDa	10%
25–100 kDa	8%

What is the optimal protein size range for a 12.5 gel?

10–70 kDa
Load 20–40 µg total protein per mini-gel well. The gels should be submerged in migration buffer normally containing SDS, except in native gel electrophoresis….

Protein size	Gel acrylamide percentage
12–45 kDa	15%
10–70 kDa	12.5%
15–100 kDa	10%
25–200 kDa	8%

What percentage is Western gel?

SDS-PAGE Gel Electrophoresis Protocol

Protein Size	Gel Percentage
12-45 kDa	15%
10-70 kDa	12.5%
15-100 kDa	10%
50-200 kDa	8%

What do gel percentages mean?

%T indicates the relative pore size of the resulting polyacrylamide gel: higher %T refers to a larger polymer-to-water ratio and on average smaller pores. The practical ranges for monomer concentration are stock solutions of 30-40%, with different ratios of acrylamide monomer to crosslinker.

How do you choose gel concentration?

WHAT’S THE BEST PERCENTAGE GEL FOR YOUR APPLICATION?

Size range between. proteins is narrow. 40–200 kD. (7.5%) 30–150 kD. (10%) 20–120 kD. (12%) Single Percentage Gels.
Size range between. proteins is broad. 10–200 kD. (4–20%) 10–100 kD. (Any kD) 20–250 kD. (4–15%) Gradient Gels.

How much protein should I load on a gel?

Standard gel combs

	Recommended loading volume*	Maximum protein load per band
Well format	1.0 mm thickness
10-well	25 µL	0.5 µg
12-well	20 µL	0.5 µg
15-well	15 µL	0.5 µg

What percentage gel should I use?

Use a high percentage agarose gel. Between 2.00% and 3.00% should help. Higher concentration gels have a better resolving power.

What percent gel would you use to resolve a 400 kDa protein?

All Answers (1) Generally To resolve a protein of molecular weight 400KDa, 7% Resolving SDS-PAGE Gel is sufficient.

Why does SDS PAGE have two gels?

So the stacking gel ensures that all of the proteins arrive at the running gel at the same time so proteins of the same molecular weight will migrate as tight bands.

How do you know when to stop running the gel?

When the dye front is nearly at the bottom of the gel it is time to stop the run. For low percent gels with a tight dye front, the dye should be on the verge of running off the gel.