In some occasions, you will have to write an essay in the extremely short amount of time on the exam in college or high school. Also, you may be a little bit of a procrastinator, and find yourself in a situation when the paper is due tomorrow morning, and you have not even chosen the topic yet. Even though a last-minute essay cannot look as great as a work prepared successively and carefully within the whole time given, you still have a chance to submit a decent paper. The working process will require your full attention and a lot of effort, even if you are assigned a simple essay. However, if you learn the next few tips, the essay writing will seem significantly easier and feasible even when you are short on time.

Firstly, clean up your working space to get started. Make sure you have everything you need on the table, take a pen, a few sticky notes, your laptop, and read through the assignment requirements. In case no prompt is given, search for good essay topics, and pick a few uncommon and interesting ones you will be able to write about. Making a final choice, think which topic is the most relevant to your current studies and will not take too much to research.

Afterwards, look for the most trustworthy sources or the ones you are certainly allowed to use. If you are not sure, access the online library or any free services where you can look for the books and articles for your essay. Use sticky notes to write down the information and put them in front of you to see how much data has been gathered and if you need to continue researching. Reread these notes from time to time and cross out the info you do not find relevant anymore.

When you have the data you need to produce a quality work, it is crucial to think about the structure of the future paper. If you are not sure how to write an essay outline properly, check what your essay type is first. Each type is organized differently, so you need to look up the structure every time you are given an essay homework. You can also search for an example of the essay on your topic, and adhere to its outline. No matter what kind of essay you are going to write, it is important to start with a thesis statement. It should declare what problem you will review in the paper, and which facts or arguments you will use to do it professionally. As these arguments will be discussed in the main part of the essay, outline the body paragraphs and put down a few sentences with the rough description of each paragraph. Think of the way you will engage the reader in the introduction, and which thought will be conclusive for the paper. When the direction of the work is clear from the outline, use it to draft the first version of the essay.

If you are not used to model essay writing, do not worry - your draft should not necessarily look like a masterpiece. It is only the depiction of your thoughts, and as you will have them written down, it will be easier to create a good essay. There is no best way to write an essay, so trust the working methods you usually use. You may like taking short breaks once in a few minutes, or write everything in one sit - just make sure to keep the focus on writing and avoid the urge to call a friend or watch something online. Thus, you will finish the paper faster, and will not feel guilty for engaging in other activities afterwards.

Do not forget to go through the essay a few times after the completion. Everyone makes typos and mistakes by accident, but it is about you to find and fix them before your teacher does. If you need help with an essay editing, try asking a friend or a family member to read and analyze your work. Also, you can order editing services in case your paper needs to be perfectly polished so that you can submit an ideal essay and get an excellent grade.

As these steps are simple to follow, you will not have any problems coping with an essay on time. Try the whole procedure at least once, and you will not have to use any other tips preparing an essay paper during your studies!

Which type of vector is pBluescript?

pBluescript is an example of a combination between plasmids and phages (phagemids). Phagemids represent a hybrid type of class of vectors that serve to produce single-stranded DNA. pBluescript is an example of a combination between plasmids and phages (phagemids).

Is pBluescript an expression vector?

The pBluescript II phagemids (plasmids with a phage origin) are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and gene mapping.

Why is pBluescript a good vector?

Agilent pBlueScript II Vectors are powerful cloning vectors for a range of research applications. Featuring an extensive polylinker with 21 unique restriction enzyme recognition sites, the vectors are suitable for a range of DNA sequencing and cloning processes.

What is E coli cloning vector?

This chapter discusses the plasmids of Escherichia coli (E. coli) as cloning vectors. The essence of molecular cloning or recombination in vitro is the joining together in vitro of two or more deoxyribonucleic acid (DNA) fragments.

What is pBluescript SK?

Plasmid: pBluescript SK (-) Standard cloning vector (phagemid excised from lambda ZAP). The f1 (-) orientation allows rescue of antisense strand ssDNA.

What is pUC18 plasmid?

pUC18 is a commonly used plasmid cloning vector in E. The molecule is a double-stranded circular DNA (2686 base pairs in length). Due to a small size pUC18 enables successful cloning of large DNA fragments.

What is shuttle vector example?

A vector that can replicate in more than one host organisms or two different cell types (e.g. a prokaryotic cell and a eukaryotic cell). An example is the yeast shuttle vector that can propagate within the cells of E. coli and yeast.

What is the size of pBluescript?

Plasmid: pBluescript SK (+)

Source/Vendor: Stratagene
Plasmid Type: Bacterial Expression
Cloning Method: Restriction Enzyme
Size: 2958
5′ Sequencing 1 Primer: M13pUC-fwd

What is E. coli plasmid?

Escherichia coli, perhaps the most studied of microorganisms, has been found to possess a variety of plasmid types. Included among these are plasmids associated with virulence. Several types of E. coli virulence plasmids exist, including those essential for the virulence of enterotoxigenic E.

What type of vector is pUC18?

pUC18 is a small, high copy cloning vector for replication in E. coli. It has been constructed using the ampicillin resistance gene and the pMB1 origin of replication from pBR322. The pMB1 of pUC18 differs from the pBR322 origin by a single point mutation and the lack of the rop gene, leading to a high copy number.

Why do we use pUC18?

Vectors pUC18 and pUC19 are small high-copy number plasmids that are widely used for cloning and manipulation of DNA fragments (9).

Is pBR322 a shuttle vector?

Most expression vectors for extrachromosomal protein expression and shuttle vectors contain the pBR322 origin of replication, and fragments of pBR322 are very popular in the construction of intraspecies shuttle or binary vectors and vectors for targeted integration and excision of DNA from chromosome.

Is the pBluescript II SK+ vector available from Addgene?

This vector is NOT available from Addgene. Standard cloning vector (phagemid excised from lambda ZAPII). The f1 (+) orientation allows rescue of sense strand ssDNA. pBluescript II SK (+) and pBluescript II KS (+) differ by the orientation of the MCS.

How are cloned genes inserted into pBluescript vectors?

With 21 restriction sites, in two orientations, most cloned genes can be inserted directionally into the pBluescript polylinkers. Polymerase chain reaction (PCR)-amplified fragments from RNA, cloned DNA, or genomic DNA can be inserted directly into pBluescript vectors, bypassing the need to construct large libraries.

Does pBluescript plasmid DNA retain its supercoiled state during DNA synthesis?

It is crucial to verify that the pBluescript plasmid DNA retains its supercoiled state during DNA synthesis. The analysis provides assurance that extensive DNA synthesis is mediated via D-loop migration and not through a DNA relaxation or rolling circle mechanism that could result from a contaminating topoisomerase or endonuclease activity]