Which type of vector is pBluescript?
pBluescript is an example of a combination between plasmids and phages (phagemids). Phagemids represent a hybrid type of class of vectors that serve to produce single-stranded DNA. pBluescript is an example of a combination between plasmids and phages (phagemids).
Is pBluescript an expression vector?
The pBluescript II phagemids (plasmids with a phage origin) are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and gene mapping.
Why is pBluescript a good vector?
Agilent pBlueScript II Vectors are powerful cloning vectors for a range of research applications. Featuring an extensive polylinker with 21 unique restriction enzyme recognition sites, the vectors are suitable for a range of DNA sequencing and cloning processes.
What is E coli cloning vector?
This chapter discusses the plasmids of Escherichia coli (E. coli) as cloning vectors. The essence of molecular cloning or recombination in vitro is the joining together in vitro of two or more deoxyribonucleic acid (DNA) fragments.
What is pBluescript SK?
Plasmid: pBluescript SK (-) Standard cloning vector (phagemid excised from lambda ZAP). The f1 (-) orientation allows rescue of antisense strand ssDNA.
What is pUC18 plasmid?
pUC18 is a commonly used plasmid cloning vector in E. The molecule is a double-stranded circular DNA (2686 base pairs in length). Due to a small size pUC18 enables successful cloning of large DNA fragments.
What is shuttle vector example?
A vector that can replicate in more than one host organisms or two different cell types (e.g. a prokaryotic cell and a eukaryotic cell). An example is the yeast shuttle vector that can propagate within the cells of E. coli and yeast.
What is the size of pBluescript?
Plasmid: pBluescript SK (+)
Source/Vendor: | Stratagene |
---|---|
Plasmid Type: | Bacterial Expression |
Cloning Method: | Restriction Enzyme |
Size: | 2958 |
5′ Sequencing 1 Primer: | M13pUC-fwd |
What is E. coli plasmid?
Escherichia coli, perhaps the most studied of microorganisms, has been found to possess a variety of plasmid types. Included among these are plasmids associated with virulence. Several types of E. coli virulence plasmids exist, including those essential for the virulence of enterotoxigenic E.
What type of vector is pUC18?
pUC18 is a small, high copy cloning vector for replication in E. coli. It has been constructed using the ampicillin resistance gene and the pMB1 origin of replication from pBR322. The pMB1 of pUC18 differs from the pBR322 origin by a single point mutation and the lack of the rop gene, leading to a high copy number.
Why do we use pUC18?
Vectors pUC18 and pUC19 are small high-copy number plasmids that are widely used for cloning and manipulation of DNA fragments (9).
Is pBR322 a shuttle vector?
Most expression vectors for extrachromosomal protein expression and shuttle vectors contain the pBR322 origin of replication, and fragments of pBR322 are very popular in the construction of intraspecies shuttle or binary vectors and vectors for targeted integration and excision of DNA from chromosome.
Is the pBluescript II SK+ vector available from Addgene?
This vector is NOT available from Addgene. Standard cloning vector (phagemid excised from lambda ZAPII). The f1 (+) orientation allows rescue of sense strand ssDNA. pBluescript II SK (+) and pBluescript II KS (+) differ by the orientation of the MCS.
How are cloned genes inserted into pBluescript vectors?
With 21 restriction sites, in two orientations, most cloned genes can be inserted directionally into the pBluescript polylinkers. Polymerase chain reaction (PCR)-amplified fragments from RNA, cloned DNA, or genomic DNA can be inserted directly into pBluescript vectors, bypassing the need to construct large libraries.
Does pBluescript plasmid DNA retain its supercoiled state during DNA synthesis?
It is crucial to verify that the pBluescript plasmid DNA retains its supercoiled state during DNA synthesis. The analysis provides assurance that extensive DNA synthesis is mediated via D-loop migration and not through a DNA relaxation or rolling circle mechanism that could result from a contaminating topoisomerase or endonuclease activity]