How do you isolate endophytic fungi from plants?
Briefly, for isolation of endophytic fungi, healthy leaves were washed thoroughly with tap water followed by washing with 10% mild bio-detergent and finally with double distilled water to remove the surface debris.
How do you identify endophytic fungi?
Morphological and Molecular Identification of Plant Endophytic Fungi. The endophytic fungal genus was identified using lactophenol cotton blue staining according to the protocol followed by Barnett and Hunter [24] and Ellis [25].
Which media is used for isolation of endophytic fungus?
The primary focus of our study was that the endophytic fungi were isolated using two distinct fungal culture media: Potato Dextrose Agar (PDA), which is largely used in mycological laboratories and is derived from potato starch (Solanum tuberosum) and a recently introduced medium called Manihot Dextrose Agar (MDA) with …
Which part of plant is used for isolation of endophytic fungus?
Results: A total of ten endophytic fungi were isolated from the stem bark of the plants. Seven of these fungi were identified, which include; Aspergillus niger, Macrophomina spp., Trichoderma spp. and four different Penicillium species, while three of the isolated endophytes remained unknown.
What is the meaning of endophytic fungi?
Endophytic fungi are symbiotically associated biota of living plant tissues that induce a symptomless disease to their hosts21 and are not host-specific.
What plants have endophytic fungi?
13.3. 2 Endophytic fungi
| Plant type | Associated endophytic fungi | Plant part |
|---|---|---|
| Ginkgo biloba | Fusarium oxysporum | Leaves |
| Allium sativum | Trichoderma brevicompactum | Bulbs |
| Musa acuminata | Fusarium proliferatum, Trichoderma atroviride | Leaf, corm, pseudostem, and fruit |
| Oryza meyeriana | Xylaria striata | Roots |
What is endophytic plant?
Endophytes are an endosymbiotic group of microorganisms that colonize in plants and microbes that can be readily isolated from any microbial or plant growth medium.
Where are endophytic fungi found?
Endophytic fungi live inside of plant tissues but don’t cause any disease symptoms. They are found in all plant species including deciduous trees, shrubs, marine algae, mosses, lichens, ferns, grasses and palms.
What do you mean by endophytic fungi?
Endophytic fungi (EF) are a group of fascinating host-associated fungal communities that colonize the intercellular or intracellular spaces of host tissues, providing beneficial effects to their hosts while gaining advantages.
Why endophytic fungi are important?
Many endophyte fungi have displayed the role in producing the natural bioactive products and using to biocontrol of plant pathogens. These fungi can be used to produce natural drugs, biopesticides, and biofertilizers that lead to decrease the dangers of synthetic chemicals.