In some occasions, you will have to write an essay in the extremely short amount of time on the exam in college or high school. Also, you may be a little bit of a procrastinator, and find yourself in a situation when the paper is due tomorrow morning, and you have not even chosen the topic yet. Even though a last-minute essay cannot look as great as a work prepared successively and carefully within the whole time given, you still have a chance to submit a decent paper. The working process will require your full attention and a lot of effort, even if you are assigned a simple essay. However, if you learn the next few tips, the essay writing will seem significantly easier and feasible even when you are short on time.

Firstly, clean up your working space to get started. Make sure you have everything you need on the table, take a pen, a few sticky notes, your laptop, and read through the assignment requirements. In case no prompt is given, search for good essay topics, and pick a few uncommon and interesting ones you will be able to write about. Making a final choice, think which topic is the most relevant to your current studies and will not take too much to research.

Afterwards, look for the most trustworthy sources or the ones you are certainly allowed to use. If you are not sure, access the online library or any free services where you can look for the books and articles for your essay. Use sticky notes to write down the information and put them in front of you to see how much data has been gathered and if you need to continue researching. Reread these notes from time to time and cross out the info you do not find relevant anymore.

When you have the data you need to produce a quality work, it is crucial to think about the structure of the future paper. If you are not sure how to write an essay outline properly, check what your essay type is first. Each type is organized differently, so you need to look up the structure every time you are given an essay homework. You can also search for an example of the essay on your topic, and adhere to its outline. No matter what kind of essay you are going to write, it is important to start with a thesis statement. It should declare what problem you will review in the paper, and which facts or arguments you will use to do it professionally. As these arguments will be discussed in the main part of the essay, outline the body paragraphs and put down a few sentences with the rough description of each paragraph. Think of the way you will engage the reader in the introduction, and which thought will be conclusive for the paper. When the direction of the work is clear from the outline, use it to draft the first version of the essay.

If you are not used to model essay writing, do not worry - your draft should not necessarily look like a masterpiece. It is only the depiction of your thoughts, and as you will have them written down, it will be easier to create a good essay. There is no best way to write an essay, so trust the working methods you usually use. You may like taking short breaks once in a few minutes, or write everything in one sit - just make sure to keep the focus on writing and avoid the urge to call a friend or watch something online. Thus, you will finish the paper faster, and will not feel guilty for engaging in other activities afterwards.

Do not forget to go through the essay a few times after the completion. Everyone makes typos and mistakes by accident, but it is about you to find and fix them before your teacher does. If you need help with an essay editing, try asking a friend or a family member to read and analyze your work. Also, you can order editing services in case your paper needs to be perfectly polished so that you can submit an ideal essay and get an excellent grade.

As these steps are simple to follow, you will not have any problems coping with an essay on time. Try the whole procedure at least once, and you will not have to use any other tips preparing an essay paper during your studies!

How do you make a 30% glycerol solution?

Prepare a solution of 30% glycerol (v/v) by mixing 30 ml of glycerol with 70 ml of water. Transfer the solution to a screw cap glass bottle and sterilize by autoclaving at 121°C for 15 min.

How do you make a 20% glycerol solution?

I prepared 80 ml of MRS broth by adding 4.176g of MRS broth to 80 ml distilled water. Then I added 20 ml of glycerol, so the amount is 100 ml.

How do you make 40 percent glycerol?

1. Measure out 40g of glycerol powder and add to a 250ml bottle. 2. Add 100ml of ddH​2​ O to the bottle.

How do you make 80 glycerol solution?

2. After you have bacterial growth, add 750 µL of the overnight culture to 250µL of 80% glycerol in a 2 mL screw top tube or cryovial and gently mix. Note: Make the 80% glycerol solution by diluting 100% glycerol in dH20 and sterilize by autoclaving.

How do you make a 5% glycerol solution?

  1. Given: Volume of solution= 20 mL; % v/v= 5% glycerol.
  2. Required: Volume of solute= __
  3. Equation: % v/v = Volume of solute/Volume solution x 100.
  4. Volume of solute = (% v/v) ( Volume solution)/ 100.
  5. Solution: Volume of solute = (20mL) (5%)/100 = 1 mL.
  6. Answer: Volume of glycerol= 1 mL glycerol then add 19 mL water.

How do you make a 10% glycerol solution?

To prepare a 10% (v/v) solution: Dilute 1 volume of molecular-biology-grade glycerol in 9 volumes of sterile pure H2O. Sterilize the solution by passing it through a pre-rinsed 0.22-μm filter.

Can you autoclave 50 glycerol?

I always autoclave the glycerol (50%). Put 0.8ml of this and 1ml of culture into the sterile tube, mix well and place at -80oC. I never sterilise the glycerol, and I make glycerol stocks on the bench, in ordinary tubes with ordinary pipette tips.

Can you autoclave 80% glycerol?

Yes, I have sterilised glycerol in the autoclave in a glassware. It works fine!

How do you prepare 10 glycerol?

To prepare a 10% (v/v) solution: Dilute 1 volume of molecular-biology-grade glycerol in 9 volumes of sterile pure H2O. Sterilize the solution by passing it through a pre-rinsed 0.22-μm filter. Store in 200-mL aliquots at 4°C.

What is 1g 100ml?

1% = 1 g in 100 ml ( =1000mg in 100ml = 10mg in 1 ml)

Is glycerol and glycerin same?

Glycerol (/ˈɡlɪsərɒl/), also called glycerine in British English and glycerin in American English, is a simple polyol compound. It is a colorless, odorless, viscous liquid that is sweet-tasting and non-toxic. The glycerol backbone is found in lipids known as glycerides.

Do I need to autoclave glycerol?

Sterilize glycerol by autoclaving for 20 minutes at 15 pounds per square inch (psi) (1.05 kg/cm2) on liquid cycle. To 1.5 ml of bacterial culture, add 0.5 ml of the sterile glycerol in a labeled storage tube (final glycerol concentration of 15%).

How do you make 50% glycerol solution?

Note: Make the 50% glycerol solution by diluting 100% glycerol in dH20. Note: Snap top tubes are not recommended as they can open unexpectedly at -80°C. Freeze the glycerol stock tube at -80°C. The stock is now stable for years, as long as it is kept at -80°C.

How much glycerol should I use to prepare a bacterial culture?

Some scientists prefer to use a glycerol solution as low as 15-40% in order to avoid compromising the bacterial culture. However, a 50% mixture will provide maximum longevity in storage. Transfer 50 microliters of the 50% glycerol solution to a microfuge tube. Move the diluted glycerol over to the new vial carefully to avoid spills.

What is the optimal concentration of glycerol for long-term storage?

The optimal concentration of long-term glycerol storage is unknown. Most labs store bacteria in 15-25% glycerol. You can prepare the glycerol stock the same time you prepare your plasmid DNA. In the morning, when you retrieve your liquid bacterial culture, take 500 μL of culture to make your glycerol stock before you begin your plasmid mini-prep.

What is the best way to prepare glycerin?

For this reason, it’s best performed by a trained technician in a controlled laboratory setting. Dilute pure glycerol in distilled water to create a 50% glycerol solution. Use a sterile pipette to measure out 10 mL of both liquids and combine them in a single flask. Stir or shake the flask thoroughly until the liquids are evenly mixed.