How do you make a 30% glycerol solution?
Prepare a solution of 30% glycerol (v/v) by mixing 30 ml of glycerol with 70 ml of water. Transfer the solution to a screw cap glass bottle and sterilize by autoclaving at 121°C for 15 min.
How do you make a 20% glycerol solution?
I prepared 80 ml of MRS broth by adding 4.176g of MRS broth to 80 ml distilled water. Then I added 20 ml of glycerol, so the amount is 100 ml.
How do you make 40 percent glycerol?
1. Measure out 40g of glycerol powder and add to a 250ml bottle. 2. Add 100ml of ddH2 O to the bottle.
How do you make 80 glycerol solution?
2. After you have bacterial growth, add 750 µL of the overnight culture to 250µL of 80% glycerol in a 2 mL screw top tube or cryovial and gently mix. Note: Make the 80% glycerol solution by diluting 100% glycerol in dH20 and sterilize by autoclaving.
How do you make a 5% glycerol solution?
- Given: Volume of solution= 20 mL; % v/v= 5% glycerol.
- Required: Volume of solute= __
- Equation: % v/v = Volume of solute/Volume solution x 100.
- Volume of solute = (% v/v) ( Volume solution)/ 100.
- Solution: Volume of solute = (20mL) (5%)/100 = 1 mL.
- Answer: Volume of glycerol= 1 mL glycerol then add 19 mL water.
How do you make a 10% glycerol solution?
To prepare a 10% (v/v) solution: Dilute 1 volume of molecular-biology-grade glycerol in 9 volumes of sterile pure H2O. Sterilize the solution by passing it through a pre-rinsed 0.22-μm filter.
Can you autoclave 50 glycerol?
I always autoclave the glycerol (50%). Put 0.8ml of this and 1ml of culture into the sterile tube, mix well and place at -80oC. I never sterilise the glycerol, and I make glycerol stocks on the bench, in ordinary tubes with ordinary pipette tips.
Can you autoclave 80% glycerol?
Yes, I have sterilised glycerol in the autoclave in a glassware. It works fine!
How do you prepare 10 glycerol?
To prepare a 10% (v/v) solution: Dilute 1 volume of molecular-biology-grade glycerol in 9 volumes of sterile pure H2O. Sterilize the solution by passing it through a pre-rinsed 0.22-μm filter. Store in 200-mL aliquots at 4°C.
What is 1g 100ml?
1% = 1 g in 100 ml ( =1000mg in 100ml = 10mg in 1 ml)
Is glycerol and glycerin same?
Glycerol (/ˈɡlɪsərɒl/), also called glycerine in British English and glycerin in American English, is a simple polyol compound. It is a colorless, odorless, viscous liquid that is sweet-tasting and non-toxic. The glycerol backbone is found in lipids known as glycerides.
Do I need to autoclave glycerol?
Sterilize glycerol by autoclaving for 20 minutes at 15 pounds per square inch (psi) (1.05 kg/cm2) on liquid cycle. To 1.5 ml of bacterial culture, add 0.5 ml of the sterile glycerol in a labeled storage tube (final glycerol concentration of 15%).
How do you make 50% glycerol solution?
Note: Make the 50% glycerol solution by diluting 100% glycerol in dH20. Note: Snap top tubes are not recommended as they can open unexpectedly at -80°C. Freeze the glycerol stock tube at -80°C. The stock is now stable for years, as long as it is kept at -80°C.
How much glycerol should I use to prepare a bacterial culture?
Some scientists prefer to use a glycerol solution as low as 15-40% in order to avoid compromising the bacterial culture. However, a 50% mixture will provide maximum longevity in storage. Transfer 50 microliters of the 50% glycerol solution to a microfuge tube. Move the diluted glycerol over to the new vial carefully to avoid spills.
What is the optimal concentration of glycerol for long-term storage?
The optimal concentration of long-term glycerol storage is unknown. Most labs store bacteria in 15-25% glycerol. You can prepare the glycerol stock the same time you prepare your plasmid DNA. In the morning, when you retrieve your liquid bacterial culture, take 500 μL of culture to make your glycerol stock before you begin your plasmid mini-prep.
What is the best way to prepare glycerin?
For this reason, it’s best performed by a trained technician in a controlled laboratory setting. Dilute pure glycerol in distilled water to create a 50% glycerol solution. Use a sterile pipette to measure out 10 mL of both liquids and combine them in a single flask. Stir or shake the flask thoroughly until the liquids are evenly mixed.