How do you make gentamicin stock solution?

For a stock solution of 7.5 mg/mL:

1. 150 mg Gentamicin.
2. 20 mL ddH2O.
3. Filter sterilize.

What is gentamicin used for in cell culture?

Gentamicin acts by binding to the 30S subunit of the bacterial ribosome leading to inhibition of protein synthesis and death in susceptible bacteria. Gibco™ Gentamicin is effective against a wide variety of gram-positive and gram-negative bacteria, and is used to prevent the contamination of cell cultures by bacteria.

Is gentamicin a liquid?

Gentamicin deionized water 50mg/mL, liquid, sterile-filtered, BioReagent, cell culture mammalian 1405-41-0.

How do you calculate antibiotic stock solution?

Ampicillin – add 1ml ampicillin (at 100mg/ml) per liter of agar to obtain a final concentration of 100ug/ml….Make sure units match!

1. C1V1 = C2V.
2. C = concentration; V = volume.
3. Example: You have a stock solution of A at 100mg/ml.
4. (100ug/ml)(150ml) = (100000ug/ml)(x)
5. 15000 = 100000x.

How do I make a 10 mg/ml stock solution?

To prepare a concentration of 10 µg/ml, pipette out 10 µl of the drug in a test tube using a micropipette. Then dilute it with 990 µl (making a total volume of 1 ml) of the solvent (ethanol, methanol, water etc) you are going to use in your experiment. Similarly, do for 20 µg/ml.

How much gentamicin do I add to cell culture?

50 µg/ml
The general recommended working concentration is 50 µg/ml for eukaryotic cell culture and 15 µg/ml for prokaryotic cells. For Laboratory Use Only. Not for drug, household or other uses. This product is recommended for use in cell culture applications at a volume of 1 ml per liter.

How much gentamicin is in cell culture?

0.5 to 50 µg /mL
Gibco™ Gentamicin is effective against a wide variety of gram-positive and gram-negative bacteria, and is used to prevent the contamination of cell cultures by bacteria. The recommended working concentration ranges from 0.5 to 50 µg /mL.

What kind of bacteria does gentamicin treat?

Gentamicin is active against a wide range of bacterial infections, mostly Gram-negative bacteria including Pseudomonas, Proteus, Escherichia coli, Klebsiella pneumoniae, Enterobacter aerogenes, Serratia, and the Gram-positive Staphylococcus.

How do you calculate antibiotic concentration?

Make sure units match!

1. C1V1 = C2V.
2. C = concentration; V = volume.
3. Example: You have a stock solution of A at 100mg/ml. You want 150 ml at 100ug/ml.
4. (100ug/ml)(150ml) = (100000ug/ml)(x)
5. 15000 = 100000x.
6. x = 0.15ml, or 150ul.
7. Remember that you have to convert mg/ml to ug/ml, which you can do by multiplying by 1000.

How do you prepare a stock solution with mg mL?

Weigh out 10mg of the extract and dissolve in 10ml of your solvent. Now take 0.1(100ul) of your stock solution and 0.9(900ul) of your solvent, this will become 1mg/ml solution.

How do you dilute mg/mL to UG mL?

mg/mL↔ug/mL 1 mg/mL = 1000 ug/mL.