What have HEK 293 cells been used for?
HEK 293 cells have been widely used in cell biology research for many years, because of their reliable growth and propensity for transfection. They are also used by the biotechnology industry to produce therapeutic proteins and viruses for gene therapy as well as safety testing for a vast array of chemicals.
What are HEK 293 cells?
HEK293T cells are a popular derivative of the original HEK293 parent cell line. The HEK293T genome contains the SV40 large T antigen, which enables them to produce recombinant proteins within plasmid vectors containing the SV40 promoter. For this reason, HEK293T is commonly used for retroviral production.
Who developed HEK293?
25, 2020 /CNW/ – The National Research Council of Canada (NRC) today formally recognized the scientific impact of the HEK 293 cell line, originally created by Dr. Frank Graham nearly 50 years ago, and its importance in the production of biologic molecules, including current COVID-19 vaccine product candidates.
Who developed HEK 293?
Why is it called HEK293?
The transformation was executed by Frank Graham, another scientist Van der Eb’s lab who invented the calcium phosphate method for transfecting cells. The source of the cells was a healthy aborted fetus of unknown parenthood. The name HEK293 is thusly named because it was Frank Graham’s 293rd experiment.
How was HEK293 immortalized?
The HEK293 cell line was originally immortalized by the random integration of viral genomic DNA of adenovirus 518, which includes the E1A and E1B genes.
Are HEK293 cells immortal?
HEK 293 immortal cell line that is comparatively easy to handle. An immortalised cell line is a population of cells from a multicellular organism which would normally not proliferate indefinitely but, due to mutation, have evaded normal cellular senescence and instead can keep undergoing division.
Why do viruses have 293T cells?
The reason for the preference of HEK293T cells is that the presence of the SV40 T-antigen in the producer cells renders them more efficient for vector production. In addition, HEK293T cells show increased cell growth and transfection efficiency in comparison to HEK293 cells.