What is allele-specific PCR used for?
Allele-specific polymerase chain reaction (AS-PCR) is a technique based on allele-specific primers, which can be used to analyze single nucleotide polymorphism (SNP) effectively including the transition, transversion and insertion/deletion polymorphism and has been exploited in the study of diseases research, molecular …
Can PCR be used for species identification?
The sensitivity of the PCR assay was at least 100 pg DNA/reaction, which was sufficient for the detection of each species of DNA. Furthermore, the nested PCR method was able to identify the species in the interspecies mixture of DNA.
What is sequence specific PCR?
HLA typing by sequence-specific primers (PCR-SSP) is a commonly used technique in HLA typing in which multiple pairs of cis-located allele-specific primers are used to determine the alleles present in a given DNA sample.
What are the different species of PCR?
Types of PCR
- Real-time PCR.
- Quantitative real time PCR (Q-RT PCR)
- Reverse Transcriptase PCR (RT-PCR)
- Multiplex PCR.
- Nested PCR.
- Long-range PCR.
- Single-cell PCR.
- Fast-cycling PCR.
What is allele specific amplification?
Allele-specific polymerase chain reaction (AS-PCR), also known as amplification refractory mutation system (ARMS) or PCR amplification of specific alleles (PASA) is a PCR-based method which can be employed to detect the known SNPs [7]. The concept of AS-PCR was initiated by Newton et al.
What are allele specific primers?
Allele Specific Primer Extension (ASPE) is a solution based, sequence specific enzymatic reaction technology that can be used to assay multiple SNPs in a single tube.
Why is PCR used for DNA barcode?
PCR Cloning Combined With DNA Barcoding Enables Partial Identification of Fish Species in a Mixed-Species Product. DNA barcoding is a valuable tool for regulatory identification of fish species; however, it does not perform well when multiple species are present within the same food product.
Why is PCR used in DNA barcoding?
DNA barcoding involves the production of PCR amplicons from particular regions to sequence them and these sequence data are used to identify or “barcode” that organism to make a distinction from other species (Lebonah et al., 2014).
What are species specific primers?
Species-specific primer pairs that produce a single band of known and different product size have been developed for each member of the clade with the exception of S. pastorianus, which is a polyphyletic allopolyploid hybrid only found in lager breweries, and for which signature sequences could not be reliably created.
How does sequence specific primer PCR differ from traditional PCR?
The main difference between PCR primers and sequencing primers is that the PCR primers are important for PCR amplification to obtain an amplicon, whereas the sequencing primers are important for sequencing a DNA fragment to reveal its nucleotide sequence.
What are the different types of PCR and what are their specific uses?
Types of polymerase chain reaction (PCR): Reverse-Transcriptase (RT–PCR) – creates complementary DNA (cDNA) by reverse transcribing RNA to DNA using reverse transcriptase. Multiplex PCR – uses a number of primers to multiply multiple fragments in a single DNA sample.
What is the difference between conventional PCR and multiplex PCR?
In conventional singleplex PCR, a single target is amplified in a single reaction tube. In contrast, multiplex PCR allows for simultaneous amplification of multiple target sequences in a single tube using specific primer sets in combination with probes labeled with spectrally distinct fluorophores.