What is BAC end sequencing?
The information about the end sequence of the bacterial artificial chromosome (BAC) clones is currently used in a wide array of applications from genome sequencing to gene discovery. The BAC end sequences of individual clones with insert DNA are collected in the large databases that can be accessed by the researcher.
What does end sequencing of BAC clones provide?
Sequencing the ends of BAC clones is an efficient strategy to produce low-pass genomic sequences, which are used to estimate genome properties such as genome organization and composition, to identify macro- and micro-synteny between species, to facilitate the assembly of contigs into scaffolds during whole genome …
What is a BAC genomic library?
Bacterial artificial chromosome (BAC) libraries are the large DNA insert libraries of choice and valuable tools for the map-based cloning of target quantitative trait loci, physical mapping, molecular cytogenetics and comparative genomics.
How is BAC different from plasmid?
Bacterial Artificial Chromosomes (BAC) have been developed to hold much larger pieces of DNA than a plasmid can. BAC vectors were originally created from part of an unusual plasmid present in some bacteria called the F’ plasmid.
What is a BAC vector?
BAC vectors are plasmids constructed with the replication origin of E. coli F factor, and so can be maintained in a single copy per cell. These vectors can hold DNA fragments of up to 300 kb.
How are BAC libraries made?
Making a BAC library For animal and human BAC libraries the DNA normally comes from white blood cells. These isolated cells are then mixed with hot agarose, a jelly-like substance. The whole mixture is poured into a mould to produce a set of small blocks, each containing thousands of the isolated cells.
What is BAC used to determine?
Blood alcohol concentration (BAC) is the metric used in measuring how much alcohol is in your bloodstream. More specifically, BAC measures how much alcohol per 100 milliliters of blood. If you have a blood alcohol level of 0.05, that means that there is 50 mg per dL of alcohol in your system, or 0.05%.
How do you calculate BAC clones?
Finding and Ordering BAC Clones
- Go to [UCSC Genome Browser]
- Click on Genome Browser.
- Select Mouse (or whatever species you need) under genome.
- Type gene name in the search box.
- Select appropriate item from search results.
- Adjust tracks as necessary, being sure to include BAC End Pairs under Mapping and Sequence Tracks.
Why is BAC and AC used in gene cloning?
Hence, both YAC and BAC are artificial DNA constructs that can maintain large human and plant genomic fragments (> 300 kb) with a high degree of stability and thus, are used to construct genome libraries and in gene transfer.
What is true BAC?
In California, you will test as legally impaired at . 08% BAC if you are over 21. It is illegal to drive or bike at this level. Significant impairment of motor coordination and loss of judgment.