What is BALB 3T3?
Cell lines developed from disaggregated BALB/c mouse embryos. They are extremely sensitive to CONTACT INHIBITION, and highly susceptible to transformation by SV40 VIRUS and murine sarcoma virus (SARCOMA VIRUSES, MURINE). Year introduced: 2004.
What are 3T3 fibroblasts?
CRL-1658™ NIH/3T3 is a fibroblast cell line that was isolated from a mouse NIH/Swiss embryo. This cell line is highly sensitive to sarcoma virus focus formation and leukemia virus propagation and has proven to be very useful in DNA transfection studies. Animal cells. Mus musculus, mouse.
What are NIH 3T3 cells used for?
The NIH/3T3 cell line is one of the most frequently used lines in material/cell interaction research and has been previously used for cytotoxicity testing of PANI [15,16,17]. Thus, the results provided by these tests can easily be compared with data published in the literature.
What is 3T3 L1 cells?
3T3-L1 is a fibroblast that was isolated from the embryo of a mouse. This cell line can be used to study the basic cellular mechanisms associated with diabetes, obesity, and related disorders. Animal cells. Mus musculus, mouse.
What are HT 29 cells?
HT-29 is a human colorectal adenocarcinoma cell line with epithelial morphology. These cells are sensitive to the chemotherapeutic drugs 5-fluorouracil and oxaliplatin, which are standard treatment options for colorectal cancer.
Are 3T3 cells diploid?
NIH 3T3 is an aneuploid cell line, but for convenience is called “diploid”.
Where do 3T3 cells come from?
The original 3T3 cell line (3T3-Swiss albino) was established in 1962 by two scientists then at the Department of Pathology in the New York University School of Medicine, George Todaro and Howard Green. Todaro and Green originally obtained their 3T3 cells from Swiss albino mouse embryo tissue.
What are 3T3-L1 fibroblasts?
What is A549 cells?
A549 cells are lung carcinoma epithelial cells that constitute a cell line. A549 is one of the commonly used cell lines for a wide range of research applications.
What are 3T3 L1 cells?
Is the balb/3t3 clone A31 a suitable transfection host?
This cell line is a suitable transfection host. The BALB/3T3 clone A31 is one of several cell lines (see ATCC CCL-164, BALB/3T12) developed by S.A. Aaronson and G.T. Todaro in 1968 from disaggregated 14- to 17-day-old BALB/c mouse embryos. modal number = 78; range = 62 to 109.
What is the number of chromosomes in balb/3t3 A31?
The BALB/3T3 clone A31 is one of several cell lines (see ATCC CCL-164, BALB/3T12) developed by S.A. Aaronson and G.T. Todaro in 1968 from disaggregated 14- to 17-day-old BALB/c mouse embryos. modal number = 78; range = 62 to 109. The stemline number is hypotetraploid. Most of the cells contained only telocentric or acrocentric chromosomes.
Is the SV40 gene function in BALB/c-3t3 cells temperature sensitive?
Thus, the viral function that induces replicative DNA synthesis in Balb/c-3T3 cells is not t and is not temperature sensitive. This SV40 gene function overrides the cellular requirement for hormonal growth factors. It does not induce transient centriole deciliation, a hormonally regulated event.
What are the products of fibroblasts?
These cells produce a diverse group of products including collagen type I, III, and IV, proteoglycans, fibronectin, laminins, glycosaminoglycans, metalloproteinases, and even prostaglandins. In the adult body, fibroblasts remain in a quiescent form until stimuli activate protein synthesis and contractile mechanisms.