What is FITC dextran?
FITC-dextran is used extensively in microcirculation and cell permeability research utilizing microfluorimetry. 22,23. FITC-dextran has been used to study plant cell wall porosity24 and capillary permeability. 25,26. Plasma proteins have been shown not to bind to FITC-dextran.
What does dextran label?
The 500,000 MW and 2,000,000 MW dextrans are labeled with fluorescein, tetramethylrhodamine or Texas Red dyes or with biotin, and all contain aldehyde-fixable lysine groups.
What is fluorescein isothiocyanate dextran?
Fluorescein isothiocyanate dextran (FITC-d) is a 3–5 kDa marker used to measure tight junction permeability.
How do you make a FITC dextran solution?
To make 100 mL solution (10 mg/mL): 10,000 Da FITC labeled dextran – 1 g. Cold 0.1M PBS pH 7.4 – 100 mL.
How do I store my FITC dextran?
Storage and stability FITC-dextran is stable for more than 6 years when stored dry in well-sealed containers at ambient temperature. Studies at 37°C in rabbit plasma, muscle homogenate, liver homogenate and urine established that FITC-dextran is stable for at least 3 days.
What is FITC in flow cytometry?
Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications including flow cytometry. First described in 1942, FITC is the original fluorescein molecule functionalized with an isothiocyanate reactive group (−N=C=S), replacing a hydrogen atom on the bottom ring of the structure.
How do you make a FITC-dextran solution?
How do you dilute a dextran FITC?
Dissolve FITC-Dextran in sterile water at a final concentration of 20 mg/ml.
How do I store my FITC-dextran?
Can you freeze FITC-dextran?
Sterilization of fairly dilute dextran solutions (1–10 mg/mL) may be performed by filtration using 0.2 μm pore-diameter sterile filters. For long-term storage, divide the aqueous solution into aliquots and freeze at ≤–20°C. AVOID REPEATED FREEZING AND THAWING.
What is Texas red dextran?
Description: Texas red labeled Dextrans are one of Nanocs fluorescent dextran conjugates with far red fluorescence. Dextrans labeled with Texas red dye have excitation/emission wavelength at 594 nm/616 nm.
What is FITC labeling?
Fluorescein isothiocyanate (FITC) is widely used to attach a fluorescent label to proteins vi a the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.