In some occasions, you will have to write an essay in the extremely short amount of time on the exam in college or high school. Also, you may be a little bit of a procrastinator, and find yourself in a situation when the paper is due tomorrow morning, and you have not even chosen the topic yet. Even though a last-minute essay cannot look as great as a work prepared successively and carefully within the whole time given, you still have a chance to submit a decent paper. The working process will require your full attention and a lot of effort, even if you are assigned a simple essay. However, if you learn the next few tips, the essay writing will seem significantly easier and feasible even when you are short on time.

Firstly, clean up your working space to get started. Make sure you have everything you need on the table, take a pen, a few sticky notes, your laptop, and read through the assignment requirements. In case no prompt is given, search for good essay topics, and pick a few uncommon and interesting ones you will be able to write about. Making a final choice, think which topic is the most relevant to your current studies and will not take too much to research.

Afterwards, look for the most trustworthy sources or the ones you are certainly allowed to use. If you are not sure, access the online library or any free services where you can look for the books and articles for your essay. Use sticky notes to write down the information and put them in front of you to see how much data has been gathered and if you need to continue researching. Reread these notes from time to time and cross out the info you do not find relevant anymore.

When you have the data you need to produce a quality work, it is crucial to think about the structure of the future paper. If you are not sure how to write an essay outline properly, check what your essay type is first. Each type is organized differently, so you need to look up the structure every time you are given an essay homework. You can also search for an example of the essay on your topic, and adhere to its outline. No matter what kind of essay you are going to write, it is important to start with a thesis statement. It should declare what problem you will review in the paper, and which facts or arguments you will use to do it professionally. As these arguments will be discussed in the main part of the essay, outline the body paragraphs and put down a few sentences with the rough description of each paragraph. Think of the way you will engage the reader in the introduction, and which thought will be conclusive for the paper. When the direction of the work is clear from the outline, use it to draft the first version of the essay.

If you are not used to model essay writing, do not worry - your draft should not necessarily look like a masterpiece. It is only the depiction of your thoughts, and as you will have them written down, it will be easier to create a good essay. There is no best way to write an essay, so trust the working methods you usually use. You may like taking short breaks once in a few minutes, or write everything in one sit - just make sure to keep the focus on writing and avoid the urge to call a friend or watch something online. Thus, you will finish the paper faster, and will not feel guilty for engaging in other activities afterwards.

Do not forget to go through the essay a few times after the completion. Everyone makes typos and mistakes by accident, but it is about you to find and fix them before your teacher does. If you need help with an essay editing, try asking a friend or a family member to read and analyze your work. Also, you can order editing services in case your paper needs to be perfectly polished so that you can submit an ideal essay and get an excellent grade.

As these steps are simple to follow, you will not have any problems coping with an essay on time. Try the whole procedure at least once, and you will not have to use any other tips preparing an essay paper during your studies!

What is FITC dextran?

FITC-dextran is used extensively in microcirculation and cell permeability research utilizing microfluorimetry. 22,23. FITC-dextran has been used to study plant cell wall porosity24 and capillary permeability. 25,26. Plasma proteins have been shown not to bind to FITC-dextran.

What does dextran label?

The 500,000 MW and 2,000,000 MW dextrans are labeled with fluorescein, tetramethylrhodamine or Texas Red dyes or with biotin, and all contain aldehyde-fixable lysine groups.

What is fluorescein isothiocyanate dextran?

Fluorescein isothiocyanate dextran (FITC-d) is a 3–5 kDa marker used to measure tight junction permeability.

How do you make a FITC dextran solution?

To make 100 mL solution (10 mg/mL): 10,000 Da FITC labeled dextran – 1 g. Cold 0.1M PBS pH 7.4 – 100 mL.

How do I store my FITC dextran?

Storage and stability FITC-dextran is stable for more than 6 years when stored dry in well-sealed containers at ambient temperature. Studies at 37°C in rabbit plasma, muscle homogenate, liver homogenate and urine established that FITC-dextran is stable for at least 3 days.

What is FITC in flow cytometry?

Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications including flow cytometry. First described in 1942, FITC is the original fluorescein molecule functionalized with an isothiocyanate reactive group (−N=C=S), replacing a hydrogen atom on the bottom ring of the structure.

How do you make a FITC-dextran solution?

How do you dilute a dextran FITC?

Dissolve FITC-Dextran in sterile water at a final concentration of 20 mg/ml.

How do I store my FITC-dextran?

Can you freeze FITC-dextran?

Sterilization of fairly dilute dextran solutions (1–10 mg/mL) may be performed by filtration using 0.2 μm pore-diameter sterile filters. For long-term storage, divide the aqueous solution into aliquots and freeze at ≤–20°C. AVOID REPEATED FREEZING AND THAWING.

What is Texas red dextran?

Description: Texas red labeled Dextrans are one of Nanocs fluorescent dextran conjugates with far red fluorescence. Dextrans labeled with Texas red dye have excitation/emission wavelength at 594 nm/616 nm.

What is FITC labeling?

Fluorescein isothiocyanate (FITC) is widely used to attach a fluorescent label to proteins vi a the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.