What is Fontana-Masson stain used for?
Background: Fontana-Masson (FM) staining is a histopathology technique used to identify dematiaceous fungi. The result often guides initial species identification and antifungal treatment; however, there is evidence that nondematiaceous fungi might react with this stain.
What stains melanin?
Fontana’s silver nitrate method stains melanin black and other tissues are red, so that melanin can be distinguished from surrounding tissues and can be detected and localized.
How do you bleach melanin?
Melanin Bleach Protocol
- Bring sections to distilled water.
- Oxidise with potassium permanganate 5 min.
- Wash in running tap water.
- Decolourise in oxalic acid 1 min.
- Wash in running tap water.
- Counterstain nuclei with haematoxylin 1 min.
- Wash in running tap water.
- Differentiate with acid alcohol.
What is Albert stain?
Albert’s staining technique is a type of special staining technique since it is used to demonstrate a special structure in bacteria. It is chiefly used to demonstrate metachromatic granules found in Corynebacterium diphtheriae. This bacterium is responsible for the disease diphtheria.
What are the 3 types of special stains?
Special Stains
- Connective Tissue Stains.
- Microrganisms.
- Carbohydrate Stains.
- Pigments, Minerals and Cytoplasmic Granules.
- Fat Stains.
Does hydrogen peroxide reduce melanin?
As a consequence, this key enzyme, which normally starts the synthesising pathway of the colouring pigment melanin, does not function anymore. Oxidation by hydrogen peroxide not only interferes with the production of melanin, but also inhibits other enzymes that are needed for the repair of damaged proteins.
Does all skin have melanin?
Melanin is a natural skin pigment. Hair, skin, and eye color in people and animals mostly depends on the type and amount of melanin they have. Special skin cells called melanocytes make melanin. Everyone has the same number of melanocytes, but some people make more melanin than others.
What does Mucicarmine positive mean?
The principle of mucicarmine staining is based on the presence of aluminum that forms a chelating complex with carmine. This changes the charge of the carmine molecule to a positive charge which allows it to bind to low density acidic substrates such as mucins.
What is the purpose of Nigrosin?
The nigrosin component of the stain is used as a counterstain to ease visualization of the unstained live spermatozoa. This stain is also widely used to assess the morphologic characteristics of the spermatozoa. Preparations are made by depositing one drop of the supravital eosin-nigrosin stain on a prewarmed slide.
Why is Albert stain used?
Albert’s stain test is used to stain and observe metachromatic granules found in Corynebacterium diphtheriae. Albert stain is a kind of differential stain used for staining and identifying metachromatic granules. The granules appear purple-black when exposed to Albert’s stain and against the light green cytoplasm.
What is Neisser stain used for?
The Neisser stain is a microbiological stain for visualising the polar bodies in the cytoplasm of gram-positive bacteria. This staining is used to identify diphtheria bacteria. Cells are stained with methylene blue and crystal violet in acidic solution and then counterstained with chrysoidin solution.