What is HuH-7 cell line?
HuH-7 (hereafter Huh7) is a permanent cell line established from male hepatoma tissue, which was surgically removed from a 57-year-old Japanese male in 1982 (Nakabayashi et al., 1982). Huh7 and its derivatives have been used as a convenient experimental substitute for primary hepatocytes.
How do you maintain a HuH-7 cell?
Subculture Protocol for HuH-7 Renew medium 2-3 times a week, or as needed according to media pH, and maintain cell confluency between 30-90%. Do not allow cell concentration to exceed 90% confluency, otherwise cells will decrease proliferation rates. The doubling time of HuH-7 cells is 24 hours.
What is Hep3B?
1-7 [Hep 3B, Hep-3B, Hep3B] is a cell line exhibiting epithelial morphology that was isolated from liver tissue derived from an 8-year-old, Black youth with liver cancer. This cell line contains an integrated hepatitis B virus genome. Homo sapiens, human. Morphology epithelial Tissue Liver Disease.
Where do VERO cells come from?
Vero cells are derived from the kidney of an African green monkey, and are one of the more commonly used mammalian continuous cell lines in microbiology, and molecular and cell biology research.
What are calu 3 cells?
Description. Calu-3 is a non-small-cell lung cancer cell line that grows in adherent culture and displays epithelial morphology. These cells have constitutively active ErbB2/Her2 due to amplification of the ERBB2 gene.
How do you thaw HuH-7 cells?
- Pre-warm growth medium in 37 °C water bath.
- Thaw the cells in a 37 °C water bath (not completely, there should be a small visible ice clump inside the tube)
- Transfer the cells into a 15 ml centrifuge tube containing 9 ml pre-warmed growth medium.
- 7.1.
Is HepG2 cancerous?
Hep G2 (or HepG2) is a human liver cancer cell line.
Where do HepG2 cells come from?
HepG2 is an immortalized cell line consisting of human liver carcinoma cells, derived from the liver tissue of a 15-year-old Caucasian male who had a well-differentiated hepatocellular carcinoma, which is the fifth most-common cancer worldwide.
Are HepG2 cells cancerous?
What are SMMC 7721 cells?
The SMMC-7721 cell line was derived from an Asian male patient with hepatocellular carcinoma in 1977. Human hepatoma cell line SMMC-7721 expresses EGFR and VEGFR and is an invaluable tool in liver cancer research.
Why are Vero cells important?
Vero cell lines are used less frequently in biological studies than the more popular HeLa cell line, in part because this is a non-human cell line. However, Vero cell lineages are still widely used for screening purposes for bacterial toxins, viruses and for parasite studies.
What are the characteristics of HuH7 cells?
HuH-7 Characteristics. The HuH-7 cell line is an immortal cell line of epithelial-like, tumorigenic cells. These cells are adherent to the surface of flasks or plates and typically grow as 2D monolayers. Although containing many mutations and INDELS, it is worthy to note the Huh7 cells have a point mutation in the p53 gene.
What can Huh-7 cells tell us about the pathophysiology of hemochromatosis?
Huh-7 cells may represent a novel and valuable tool to investigate the role of altered HFE traffic in iron metabolism and pathogenesis of human HFE HC. Huh-7: a human “hemochromatotic” cell line Hepatology. 2010 Feb;51(2):654-9.doi: 10.1002/hep.23410. Authors
What is the Huh-7 model of hepatocellular carcinoma?
The HuH-7 CDX model of human hepatocellular carcinoma is a mouse model that enables pre-clinical tumor growth inhibition studies targeting kinase inhibitors (e.g. BZG-4000), FGFR4, anti-EGFRvIII antibodies and other novel anti-tumor growth therapeutics (e.g. sorafenib, silibinin).
What is the point mutation in the Huh7 cell line?
Although containing many mutations and INDELS, it is worthy to note the Huh7 cells have a point mutation in the p53 gene. The Huh-7 cell line (origin: human liver) is used to create the Cell Line Derived Xenograft (CDX) HuH-7 mouse model.