What is goat anti rabbit IgG?
Product Description. Goat anti rabbit IgG antibody recognizes the Fc region of rabbit immunoglobulin G, confirmed by ELISA and immunoelectrophoresis. The antibody has not been species cross adsorbed and may cross react with IgG from other species. It shows weak recognition of rabbit IgM in ELISA.
What is the role of goat anti rabbit IgG horseradish?
Goat anti-Rabbit IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Summary. Detects rabbit IgG heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with human IgG, mouse IgG, and chicken IgY is observed.
What is anti rabbit antibody?
Anti-rabbit secondary antibodies are generated by immunizing a host animal with a pooled population of immunoglobulins (Ig) from the target species. In this case, the Ig would come from rabbits and be introduced into one of the available host species: goat, donkey, mouse, chicken, or sheep.
Is Alexa Fluor an antibody?
Alexa Fluor® 488 secondary antibody is ideal immunofluorescence antibodies to get bright and sensitive immunofluorescent staining in cell and tissue fluorescent microscopy.
What is HRP conjugated secondary antibody?
Horseradish Peroxidase (HRP) has a high turnover rate that allows HRP secondary antibodies to generate strong signal in a short time span. HRP secondary antibodies are commonly used in western blot (WB), immunohistochemistry (IHC) and ELISA.
What is goat anti mouse antibody?
Anti-mouse secondary antibodies are generated by immunizing a host animal (e.g., a goat) with a pooled population of immunoglobulins (Ig) from the target species (i.e., mice). After the host animal’s immune system responds to produce anti-mouse Ig antibodies, serum is collected, and the target antibodies are purified.
Why is horseradish peroxidase important?
Horseradish peroxidase (HRP) mediates efficient conversion of many phenolic contaminants and thus has potential applications for pollution control. Such potentially important applications suffer however from the fact that the enzyme becomes quickly inactivated during phenol oxidation and polymerization.
What does goat anti-mouse antibody mean?
Whats is IgG?
Immunoglobulin G (IgG): This is the most common antibody. It’s in blood and other body fluids, and protects against bacterial and viral infections. IgG can take time to form after an infection or immunization.
How do you detect HRP?
The emitted light can be captured on an X-Ray film. This most commonly used detection method involves detection using secondary antibodies conjugated with either HRP (horseradish peroxidase) or AP (alkaline phosphatase). Horseradish Peroxidase-luminol system: Luminol is a chemiluminescent substrate of HRP.